报告题目：Genetically encoded sensors for molecular imaging and manipulating in living cell

报告人：　 Yi Yang

华东理工大学生物反应器工程国家重点实验室副主任

 上海市高校特聘教授

Synthetic Biology and Biotechnology Laboratory,

State Key Laboratory of Bioreactor Engineering,

School of pharmacy, East China University of Science and Technology

时间：       2015年04月17日（周五），下午：14:00-15:00

地点：       西区生物楼429会议室

主办单位：中国科学技术大学生命科学学院

                 合肥微尺度物质科学国家实验室

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报告简介：

During the Post-genomic Era, methodologies with quantitative, microsopic and dynamic potential are greatly desired by biomedical research and biotechnology. Particularly, spatiotemporal imaging and manipulating cellular metabolism provides indispensable tools for tackling of complex biological questions. We recently developed a series of intensely fluorescent, rapidly responsive, genetically encoded sensor of wide dynamic range, denoted SoNar for tracking cytosolic NAD+ and NADH redox states in living cells and in vivo, which respond to subtle perturbations of various pathways of energy metabolism in real-time, and allowed high-throughput screening for new agents targeting tumor metabolism. It is challenging for current regulated transgene systems, mainly the chemical inducible gene expression systems, to precisely switch on/off gene expression at an exact location and time. We have developed a easy to use and robust light switchable gene expression system, which is termed as LightOn transgene system and based on a synthetic light switchable transcription activator. The transcription activator binds promoters upon blue light exposure, and subsequently initiates transcription of target genes in mammalian cells. The fast kinetics, high transcriptional activity and reversibility of the gene expression system render it a powerful tool to tackle complex biological questions and provide a novel solution for titrated spatiotemporal control of gene therapy. We anticipate these tools will be widely used in many fields of life science research and biotechnology.